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Sunitha, N.
- Development of Validated RP-HPLC Method for the Estimation of Itraconazole in Pure and Pharmaceutical Dosage Form
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1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 3, No 4 (2013), Pagination: 119-123Abstract
A simple, fast and precise RP-HPLC method was developed for the quantification of Itraconazole in pure and pharmaceutical dosage form. The quantification was carried out using Dionex C18 4.6 X 250mm, 5μm enhanced polar selectivity column and mobile phase comprised of methanol and pH 7.5 potassium dihydrogen phosphate in the ratio of 40:60 and degassed under ultrasonication. The flow rate was 1.5ml/min and the effluent was monitored at 306nm. The retention time of Itraconazole was found to be 5.2 min. The method was validated in terms of linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation in accordance with ICH guidelines. Linearity of Itraconazole was in the range of 200-600 μg/mL. The percentage recoveries of Itraconazole were 99.33% to 99.66% from the capsule formulation. The proposed method is suitable for determination of Itraconazole in pharmaceutical dosage form.Keywords
Chromatography, Itraconazole, Method Validation.- Development and Validation of RP–HPLC Method for Simultaneous Estimation of Cefepime and Tazobactam in Injection Formulation
Abstract Views :207 |
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Authors
Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 3, No 4 (2013), Pagination: 131-137Abstract
A new, rapid, highly sensitive, economical and accurate RP-HPLC method was developed for simultaneous estimation of Cefepime and Tazobactam in injection formulation. The separation was achieved by C18 column (150 × 4.6 mm, 5 μ particle size) with mobile phase consisting of phosphate buffer (pH 2.4, diluted with orthophosphoric acid), methanol and acetonitrile in the ratio of 90:5:5 v/v, using flow rate 1.1 mL/min and eluents monitored at 260 nm. The developed method was validated as per ICH guidelines for specificity, linearity, precision, accuracy, robustness, limit of detection and limit of quantification. The retention times of cefepime and tazobactam were 2.236 and 4.498 min respectively. The linearity was found to be in the range of 250-750 μg/mL and 31.25-93.75 μg/mL for cefepime and tazobactam sodium respectively, had regression coefficients (R2) 0.999. The proposed method was successfully applied for simultaneous estimation of both drugs in injection formulation.Keywords
RP-HPLC, Cefepime, Tazobactam, Injection, Validation.- Stability Indicating RP–HPLC Method for the Estimation of Acamprosate in Pure and Tablet Dosage Form
Abstract Views :204 |
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Authors
Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN